Use of a Rapid Fluorescent-Antibody Staining Technique with Group A Streptococci

Rapid fluorescent-antibody staining technique.

The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....

Rapid fluorescent-antibody method with bromelase for identification of group A streptococci.

This is a preliminary report on a rapid fluorescent-antibody staining method utilizing the enzyme Bromelase that effectively eliminates nonspecific staining of staphylococci. By this simple method, group A streptococci were accurately identified from 2- to 4-h broth cultures.

RAPID IDENTIFICATION OF GROUP A STREPTOCOCCI BY PYRROLIDONYL-β-APHTHYLAMIDE HYDROLYSIS

In a clinical trial a new pyrrolidonyl-β-naphthylamide (PYR) hydrolysis test was compared with the bacitracin disk susceptibility test for accuracy in the presumptive identification of group A streptococci (GAS). Among 128 isolates of beta-hemolytic streptococci 93 group A isolates were found. The sensitivity of the PYR and bacitracin tests were similar (98.9%), but the bacitracin test had ...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Studies on Fluorescent Antibody Staining

1. A study has been made of the non-specific fluorescent staining of splenic imprints treated with fluorescent sheep antibody globulins. 2. In tissue imprints made with the spleens of antigen-stimulated animals, no morphological distinction was evident between areas showing non-specific fluorescence and specific fluorescence. 3. Elimination of non-specific fluorescence was not achieved by any o...